Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Targeted Delivery System Based on Gemcitabine-Loaded Silk Fibroin Nanoparticles for Lung Cancer Therapy

Abstract

Here, a targeted delivery system was developed based on silk fibroin nanoparticles (SFNPs) for the systemic delivery of gemcitabine (Gem) to treat induced lung tumor in a mice model. For targeting the tumorigenic lung tissue, SP5-52 peptide was conjugated to Gem-loaded SFNPs. Different methods were used to characterize the structural and physicochemical properties of the SFNPs. The prepared nanoparticles (NPs) showed suitable characteristics in terms of size, zeta potential, morphology, and structural properties. Moreover, the targeted Gem-loaded SFNPs showed higher cytotoxicity, cellular uptake, and accumulation in the lung tissue in comparison to the nontargeted SFNPs and control groups. Afterward, a mice model with induced lung tumor was developed by intratracheal injection of Lewis lung carcinoma (LL/2) cells into the lungs for assessing the therapeutic efficacy of the prepared drug delivery system. The histopathological assessments and single-photon-emission computed tomography-CT radiographs showed successful lung tumor induction. Moreover, the obtained results showed higher potential of targeted Gem-loaded SFNPs in treating induced lung tumor compared with that of the control groups. Higher survival rate, less mortality, and no sign of metastasis were also observed in those animals treated with targeted NPs based on the histological and radiological analyses. This study presented an effective anticancer drug delivery system for specific targeting of induced lung tumor that could be useful in treating malignant lung cancers in future.

Journal
ACS Appl. Mater. Interfaces
Volume
9
Issue
37
Pagination
31600–31611
Publisher
American Chemical Society
ISSN
1944-8252
URL
https://doi.org/10.1021/acsami.7b10408
Keywords
gemcitabine, Lewis lung carcinoma cells, lung tumor induction, Silk Fibroin, SP5-52 peptide
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Year of Publication
2017
DOI
10.1021/acsami.7b10408
Date Published
2017-08-24
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