Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Sulfated alginate as a mimic of sulfated glycosaminoglycans: Binding of growth factors and effect on stem cell behavior

Abstract

Sulfated glycosaminoglycans (GAGs) are principal elements of the extracellular matrix, where they are involved in a plethora of signaling pathways mainly via interactions with diverse proteins such as growth factors and cytokines. However, the mechanisms that drive these interactions are not yet clear, mostly
because of the difficulty to access large quantities of homogeneously sulfated natural GAGs. In this work, GAG mimics are synthesized from readily available alginate with different degrees of sulfation (DS, from 0.8 to 2.6) by simple process. The effect of the DS is determined on the binding of basic fibroblast growth factor (FGF-2). The enzyme-linked immunosorbent assay demonstrates that the binding of FGF-2 is significantly greater for alginates with high DS as compared to unmodified and low sulfated analogs. These results are
further applied to engineer FGF-2-loaded substrates for stem cell culturing using the layer-by-layer approach. All films support the attachment and growth of adipose derived stem cells (ADSCs). Noteworthy, highly sulfated alginates maintain the stemness of the ADSCs that exhibit remarkably long filopodia. These results can be exploited in the engineering of novel substrates that induce targeted cell behavior via controlled protein delivery and for tissue engineering constructs applicable in various regenerative approaches.

Journal
Advanced Biosystems
Volume
1
Issue
7
Pagination
1700043
Publisher
Wiley
ISSN
2366-7478
URL
http://onlinelibrary.wiley.com/doi/10.1002/adbi.201700043/epdf
Keywords
ALGINATE, biomimetic materials, growth factors, layer-by-layer, sulfated glycosaminoglycans
Rights
Restricted Access
Peer Reviewed
Yes
Status
published
Project
CHEM2NATURE
Year of Publication
2017
DOI
10.1002/adbi.201700043
Date Published
2017-06-16
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