Biomaterials, Biodegradables and Biomimetics Research Group

Papers in Scientific Journals

Human Adipose Tissue-Derived SSEA-4 SubPopulation Multi-Differentiation Potential Towards the Endothelial and Osteogenic Lineages


Human adipose tissue has been recently recognized as a potential source of stem cells for regenerative medicine applications, including bone tissue engineering (TE). Despite the gathered knowledge regarding the differentiation potential of human adipose tissue-derived stem cells (hASCs), in what concerns the endothelial lineage many uncertainties are still present. The existence of a cell subpopulation within the human adipose tissue that expresses a SSEA-4 marker, usually associated to pluripotency, raises expectations on the differ- entiation capacity of these cells (SSEA-4+ hASCs). In the present study, the endothelial and osteogenic dif- ferentiation potential of the SSEA-4+hASCs was analyzed, aiming at proposing a single-cell source/ subpopulation for the development of vascularized bone TE constructs. SSEA-4 + hASCs were isolated using immunomagnetic sorting and cultured either in a-MEM, in EGM-2 MV (endothelial growth medium), or in osteogenic medium. SSEA-4 + hASCs cultured in EGM-2 MV formed endothelial cell-like colonies character- ized by a cobblestone morphology and expression of CD31, CD34, CD105, and von Willebrand factor as determined by quantitative reverse transcriptase (RT)-polymerase chain reaction, immunofluorescence, and flow cytometry. The endothelial phenotype was also confirmed by their ability to incorporate acetylated low- density lipoprotein and to form capillary-like structures when seeded on Matrigel. SSEA-4 + hASCs cultured in a-MEM displayed fibroblastic-like morphology and exhibited a mesenchymal surface marker profile (>90% CD90+/CD73+/CD105+). After culture in osteogenic conditions, an overexpression of osteogenic-related markers (osteopontin and osteocalcin) was observed both at molecular and protein levels. Matrix minerali- zation detected by Alizarin Red staining confirmed SSEA-4+hASCs osteogenic differentiation. Herein, we demonstrate that from a single-cell source, human adipose tissue, and by selecting the appropriate subpop- ulation it is possible to obtain microvascular-like endothelial cells and osteoblasts, the most relevant cell types for the creation of vascularized bone tissue-engineered constructs.

Tissue Engineering part A
endothelial and osteogenic differentiation, SSEA-4+ human adipose derived stem/stromal cells
Restricted Access
Peer Reviewed
Year of Publication
Date Published
Search Google ScholarGenerate BibTexDownload RTF
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.