Peripheral nerve injuries (PNI) are a large-scale problem that affects over one million people around the world. For PNI that require surgical intervention and in the case of long gap injuries, autologous nerve grafts (ANG) have been considered the gold standard for decades. However, the random efficiency of ANG is associated to several drawbacks, for instance, donor site morbidity, limited availability and nerve mismatches, leading to merely 50% rates of success. This experimental work focuses on the development of novel Gellan Gum (GG) biomaterials for the engineering of a construct able to support peripheral nerve regeneration (PNR). This approach consists in the development of different GG systems with varying amounts of High acyl GG (HA-GG) and Methacrylated GG (MA-GG) as luminal fillers of nerve guides made of chitosan with 5% degree of acetylation (Reaxon®, Medovent, Germany). The hypothesis behind the application of GG systems is that they present varying degradation rates and add further guiding structures to the hollow chitosan tubes, for Schwann cells and axons support and migration.