Biomaterials, Biodegradables and Biomimetics Research Group

Comunications - Poster

Manganese-based Methacrylated Gellan Gum Hydrogels as Injectable System for Image-guided Cell Delivery


INTRODUCTION: Hydrogels hold a great potential on the scope of cell-based therapies and can be used to unravel several bottlenecks of these approaches [1]. Herein, we intend to develop injectable manganese-based methacrylated gellan gum hydrogels that can be monitored via MRI imaging.
METHODS: 0.75% (w/v) methacrylated gellan gum (GG-MA) solutions were supplemented with 0.1 and 1 mM of MnCl2 (Sigma-Aldrich) and further crosslinked with artificial cerebrospinal fluid (aCSF). The resulting hydrogels were characterized regarding their injectability, stability and MRI signal. As proof-of-concept, human-derived adipose stem cells (hASC) were encapsulated in 0.1 mM MnCl2 GG-MA hydrogels and cultured for 7 days. During the culture period, cell viability was assessed using Live/Dead staining kit (Thermo Fisher).
RESULTS & DISCUSSION: All the prepared formulations are injectable, with the force needed to inject gel solution being lower or similar to the force needed to inject water. After the 7 days of incubation in aCSF, hydrogel degradation was approximately 20% for all the formulations tested. T1-weighted MRI scans showed that formulation with 0.1mM MnCl2 exhibits a higher MRI signal. Therefore, in vitro studies were performed using this condition. hASCs encapsulated into hydrogel fibers remained alive up to 7 days of culture.
CONCLUSIONS: Manganese-based GG-MA hydrogels were successfully prepared. These hydrogels hold great potential for image-guided cell delivery approaches, when minimally invasive procedures are envisaged.

Cell delivery, Gellan Gum
Open Access
Peer Reviewed
Year of Publication
Date Published
Search Google ScholarGenerate BibTexDownload RTF
This website uses cookies. By using this website you consent to our use of these cookies. For more information visit our Policy Page.